STUDY NAME
GOLD Study ID Gs0056643
Study Name Human fecal viriome from healthy adults, Genome Institute of Singapore
Other Names Uncultured Human Fecal Virus Metagenome
NCBI Umbrella Bioproject Name
NCBI Umbrella Bioproject ID
Legacy ER Study ID 11643
Legacy GOLD ID Gm00055
Added By Nikos Kyrpides on 2007-11-27
Last Modified By Michelle Isbandi on 2013-12-18
STUDY DESCRIPTION
PI Name Yijun Ruan
Description The human gut is known to be a reservoir of a wide variety of microbes, including viruses. Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy humans has not been described. Here, we present a comparative metagenomic analysis of the RNA viruses found in three fecal samples from two healthy human individuals. For this study, uncultured viruses were concentrated by tangential flow filtration, and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the 36,769 viral sequences obtained were similar to plant pathogenic RNA viruses. The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations.up to 109 virions per gram of dry weight fecal matter. PMMV was also detected in 12 (66.7%) of 18 fecal samples collected from healthy individuals on two continents, indicating that this plant virus is prevalent in the human population. A number of pepper-based foods tested positive for PMMV, suggesting dietary origins for this virus. Intriguingly, the fecal PMMV was infectious to host plants, suggesting that humans might act as a vehicle for the dissemination of certain plant viruses.
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Study Information Visibility Public
GOLD CLASSIFICATION
Ecosystem Host-associated
Ecosystem Category Human
Ecosystem Type Digestive system
Ecosystem Subtype Large intestine
Specific Ecosystem Fecal
Metagenomic Study Yes
STUDY COMPOSITION
Number of Biosamples 3
Number of Seq. Projects 3
Number of Analysis Projects 3
Number of Related Studies 0